Open Internet by MindsNet

Persistent Column Fouling in HPLC Analysis of Spray-Dried Formulations

The author is experiencing persistent column fouling issues with a HPLC assay for an API spray-dried in HPMCAS, causing a stubborn peak to co-elute with the API. Despite trying various flushing methods, the issue remains unresolved. The author seeks advice on whether this is polymer fouling and the best approach for cleaning the column.

Science, Chemistry, Analytical Chemistry