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Optimizing Expression and Purification of Ornithine Decarboxylase (ODC)

The author is struggling to express and purify ornithine decarboxylase (ODC) due to its sensitivity to expression and purification conditions, resulting in inconsistent or low-yield results. They are seeking advice on common pitfalls, optimization strategies, and effective expression/purification techniques. This challenge is hindering progress in their research.

Science, Biology, Cell Biology