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Improving 16S rRNA Gene Sequencing for Microbiota Taxonomy

The author faces challenges in distinguishing between certain bacterial species using 16S rRNA gene sequencing, such as Bacillus subtilis group, Escherichia/Shigella, Bifidobacterium longum vs B. infantis vs B. suis, and Streptococcus mitis vs oralis vs pneumoniae. The current database used for EMU classification seems outdated, leading to potential inaccuracies in taxonomy assignment.

Science, Biology, Microbiology